%0 Journal Article %T Changes in enzymes activity, substrate utilization pattern and diversity of soil microbial communities under cadmium pollution %A Muhammad Akmal %A WANG Hai-zhen %A WU Jian-jun %A XU Jian-ming %A XU De-fu %A
Muhammad Akmal %A XU Jian-ming %A LI Zhao-jun %A WANG Hai-zhen %A XU De-fu %J 环境科学学报(英文版) %D 2005 %I %X Heavy metal pollution has received increasing attention in recent years mainly because of the public awareness of environmental issues. In this study we have evaluated the effect of cadmium (Cd) on enzymes activity, substrate utilization pattern and diversity of microbial communities in soil spiked with 0, 20, 40, 60, 80, and 100 mg/kg Cd, during 60 d of incubation at 25 degrees C. Enzyme activities determined at 0, 15, 30, 45, and 60 d after heavy metal application (DAA) showed marked declines for various Cd treatments, and up to 60 DAA, 100 mg/kg Cd resulted in 50.1%, 47.4%, and 39.8% decreases in soil urease, acid phosphatase and dehydrogenase activities, respectively to control. At 60 DAA, substrate utilization pattern of soil microbial communities determined by inoculating Biolog ECO plates indicated that Cd addition had markedly inhibited the functional activity of soil microbial communities and multivariate analysis of sole carbon source utilization showed significantly different utilization patterns for 80 and 100 mg/kg Cd treatments. The structural diversity of soil microbial communities assessed by PCR-DGGE method at 60 DAA, illustrated that DGGE patterns in soil simplified with increasing Cd concentration, and clustering of DGGE profiles for various Cd treatments revealed that they had more than 50% difference with that of control. %K enzyme activity %K microbial community %K soil %K cadmium
土壤污染 %K 镉污染 %K 重金属 %K 环境污染 %K 酶 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=B55455E6B58C1F09CA8FDA336F4A9651&yid=2DD7160C83D0ACED&vid=BCA2697F357F2001&iid=94C357A881DFC066&sid=4AB2A4AB67F2B16E&eid=4FBF7A066D63EF1A&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=5&reference_num=59