%0 Journal Article
%T Using the nematode Caenorhabditis elegans as a model animal for assessing the toxicity induced by microcystin-LR<br>
%A LI Yunhui
%A WANG Yang
%A YIN Lihong
%A PU Yuepu
%A WANG Dayong
%A <br>
%J 环境科学学报(英文版)
%D 2009
%I 
%X Among more than 75 variants of microcystin (MC), microcystin-LR (MC-LR) is one of the most common toxins. In this study, the feasibility of using Caenorhabditis elegans to evaluate MC-LR toxicity was studied. C. elegans was treated with MC-LR at different concentrations ranging from 0.1 to 80 μg/L. The results showed that MC-LR could reduce lifespan, delay development, lengthen generation times, decrease brood sizes, suppress locomotion behaviors, and decreases hsp-16-2-gfp expression. The endpoints of generation time, brood size, and percentage of the population expressing hsp16-2-gfp were very sensitive to 1.0 μg/L of MC-LR, and would be more useful for the evaluation of MC-LR toxicity. Furthermore, the tissue-specific hsp16-2-gfp expressions were investigated in MC-LR-exposed animals, and the nervous system and intestine were primarily effected by MC-LR. Therefore, the generation time, brood size, and hsp16-2-gfp expression in C. elegans can be explored to serve as valuable endpoints for evaluating the potential toxicity from MC-LR exposure.
%K microcystin-LR
%K toxicity assessment
%K brood size
%K stress response
%K Caenorhabditis elegans<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=409EB90638C98176D6C18EE4AF207249&yid=DE12191FBD62783C&vid=659D3B06EBF534A7&iid=38B194292C032A66&sid=396DD691E964F390&eid=238BD7580EFCC5AE&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=1&reference_num=36