%0 Journal Article
%T Effects of culture conditions on ligninolytic enzymes and protease production by Phanerochaete chrysosporium in air
%A XIONG Xiaoping
%A WEN Xianghu
%A BAI Yanan
%A QIAN Yi
%A <br>XIONG Xiaoping
%A WEN Xianghu
%A BAI Yanan
%A QIAN Yi
%J 环境科学学报(英文版)
%D 2008
%I 
%X The production of ligninolytic enzymes and protease by Phanerochaete chrysosporium was investigated under different culture conditions. Different amounts of medium were employed in free and immobilized culture, together with two kinds of medium with different C/N ratios. Little lignin peroxidase (LiP) (< 2 U/L) was detected in free culture with nitrogen-limited medium (C/N ratio: 56/2.2 mmol/L), while manganese peroxidase (MnP) maximum activity was 231 and 240 U/L in 50 and 100 ml medium culture, respectively. Immobilized culture with 50 ml nitrogen-limited medium gave the highest MnP and LiP production with the maximum values of 410 and 721 U/L separately on day 5; however, flasks containing 100 ml nitrogen-limited medium only produced less MnP with a peak value of 290 U/L. Comparatively, carbon-limited medium (C/N ratio: 28/44 mmol/L) was adopted in culture but produced little MnP and LiP. Medium type had the greatest impact on protease production. Large amount of protease was produced due to glucose limitation. Culture type and medium volume influence protease activity corporately by affecting oxygen supply. The results implied shallow immobilized culture was a possible way to gain high production of ligninolytic enzymes.
%K protease
%K culture conditions
%K ligninolytic enzymes
%K Phanerochaete chrysosporium<br>蛋白酶
%K 培养条件
%K 木质素溶菌酶
%K 生物处理
%K 废水处理
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=AC6C0B218A8E1E8B126894C88DAB9DE4&yid=67289AFF6305E306&vid=A04140E723CB732E&iid=CA4FD0336C81A37A&sid=BB0EA31DB1B01173&eid=8C83C265AD318E34&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=0&reference_num=27