%0 Journal Article
%T Relationship between ATPase activity and conjugated polyamines in mitochondrial membrane from wheat seedling roots under osmotic stress
Relationship between ATPase activity and conjugated polyamines in mito-chondrial membrane from wheat seedling roots under osmotic stress
%A LIU Huai-pan
%A LIU Jun
%A ZHANG Yan-yan
%A LIU You-liang
%A
LIU Huai-pan
%A LIU Jun
%A ZHANG Yan-yan
%A LIU You-liang
%J 环境科学学报(英文版)
%D 2004
%I
%X The effects of osmotic stress on the ATPase activity, the contents of -SH group and conjugated polyamines in mitochondrial membrane from wheat seedling Triticum aestivum L. cv. Yumai No. 18 (drought-tolerant) and cv. Yumai No. 9 (drought-sensitive)] roots were investigated. The results showed that ATPase activity and -SH group content decreased with polyethylene glycol(PEG) 6000(-0.55 MPa) treatment for 7 d, in concert with the decrease of the ratio of noncovalently conjugated spermidine (NCC-Spd)/noncovalently conjugated putrescine(NCC-Put) and increase of the covalently conjugated putrescine (CC-Put). Osmotic stress injury to Yangmai No.9 seedlings was alleviated greatly with 1 mmol/L exogenous spermidine(Spd), in concert with marked increases of the ratio of NCC-Spd/NCC-Put, -SH group contents and ATPase activity in mitochondrial membrane. Under osmotic stress, the concomitant treatment of Yumai No. 18 seedlings with methylglyoxyl bis(guanylhydrazone) (MGBG), an inhibitor of S-adenosyl methionine decarboxylase(SAMDC), and phenanthrolin (o-Phen), an inhibitor of transglutaminase(TGase), caused a significant decrease of the ratio of NCC-Spd/NCC-Put, CC-Put contents, respectively, in concert with the marked decreases of ATPase activity, -SH group content and its tolerance to osmotic stress. All the results above suggested that osmotic stress tolerance of wheat seedlings was associated with the ATPase activity, the contents of -SH group, NCC-Spd and CC-Put in mitochondrial membrane.
%K ATPase
%K conjugated polyamines
%K mitochondria
%K osmotic stress
%K wheat(Triticum aestivum L
%K )
腺苷三磷酸酶
%K ATP酶
%K 活性
%K 渗透性
%K 小麦
%K 环境污染
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=A88CB7BD310E996327F84CB9A26E2A2D&yid=D0E58B75BFD8E51C&vid=7801E6FC5AE9020C&iid=94C357A881DFC066&sid=AB720B703F452703&eid=27350781F1397F32&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=12&reference_num=18