%0 Journal Article
%T Effects of temperature on UV-B-induced DNA damage and photorepair in Arabidopsis thaliana
%A Shao-shan Li
%A Yan Wang
%A Lars Olof Bj?rn
%A <br>LI Shao-shan
%A WANG Yan
%A BJ?RN Lars Olof
%J 环境科学学报(英文版)
%D 2004
%I 
%X DNA damage in the form of cyclobutane pyrimidine dimers(CPDs) and (6-4) photoproducts(6-4PPs) induced by UV-B radiation in Arabidopsis thaliana at different temperatures was investigated using ELISA with specific monoclonal antibodies. CPDs and 6-4PPs increased during 3 h UV-B exposure, but further exposure led to decreases. Contrary to the commonly accepted view that DNA damage induced by UV-B radiation is temperature-independent because of its photochemical nature, we found UV-B-induction of CPDs and 6-4PPs in Arabidopsis to be slower at a low than at a high temperature. Photorepair of CPDs at 24 degrees C was much faster than that at 0 degrees C and 12 degrees C, with 50% CPDs removal during 1 h exposure to white light. Photorepair of 6-4PPs at 12 degrees C was very slow as compared with that at 24 degrees C, and almost no removal of 6-4PPs was detected after 4 h exposure to white light at 0 degrees C. There was evidence to suggest that temperature-dependent DNA damage and photorepair could have important ecological implications.
%K Arabidopsis thaliana
%K cyclobutane pyrimidine dimers(CPDs)
%K DNA repair
%K (6-4) photoproducts(6-4PPs)
%K temperature
%K UV-B radiation<br>UV-B辐射
%K DNA修复
%K 嘧啶化合物
%K 6-4PPs
%K CPDs
%K ELISA
%K 温度
%K 基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=451C3375354E40101DC5440F4FAB8B4F&yid=D0E58B75BFD8E51C&vid=7801E6FC5AE9020C&iid=CA4FD0336C81A37A&sid=DABEF202280E7EF1&eid=5BC9492E1D772407&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=2&reference_num=21