%0 Journal Article
%T Metabolism of N-phenyl-2-naphthylamine andN-phenyl-1-naphthylamine by rat hepaticmicrosomes and hepatocytes
%A Xue XuanxianInstitute of Cell Biology
%A Chinese Academy of Sciences
%A Shanghai
%A ChinaThomas WolffInstitute fur Toxikologie
%A Gesellchaft fur Strahlen-und Umweltforchung
%A Munchen
%A D- Neuherberg
%A Germany
%A <br>Xue Xuanxian
%A Thomas Wolff
%J 环境科学学报(英文版)
%D 1992
%I 
%X The carcinogenic antioxidants,N-phenyl-1-naphthylamine (P1NA)and N-phenyl-2-naphthylamine (P2NA) were examined in vitro for biotransformation by rat hepatic microsomes and in freshly isolated hepatocytes. HPLC-analysis of hepatocyte incubations with revealed that phenols were the major metabolites in both cases. P1NA formed one phenolic metabolite only, while incubation with P2NA yielded two phenols identified as 6-hydroxy-P2NA and 4'-hydroxy-P2NA by cochromatography with authentic samples. β-naphthylamine, a metabolite indicating dephenylation of P2NA was not detectable.Metabolism studies with microsomes revealed that the phenols were formed by cytochrome P-450 dependent monooxygenases. Pretreatment of animals with phenobarbital and 3-methylcholanthrene both increased the rate of microsomal metabolism of P1NA and P2NA, indicating that more than one P-450 enzyme mediate the oxygenation reaction. Animal pretreatment with single and repeated doses of P1NA and P2NA did not markedly stimulate metabolism, b
%K N-phenyl-2-naphthylamine
%K N-phenyl-1-naphthylamine
%K hepatocytes<br>N-phenyl-2-naphthylamine
%K N-phenyl-1-naphthylamine
%K hepatocytes.
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=2ED9F5BC1172AE05EA7C429F0B196AED&yid=F53A2717BDB04D52&vid=E158A972A605785F&iid=CA4FD0336C81A37A&sid=67969BA850333433&eid=06EA2770E96C5402&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=0&reference_num=0