%0 Journal Article
%T Effects of beta-cyclodextrins on the enzymatical hydrolysis of chiral dichlorprop methyl ester.<br>Effects of β-cyclodextrins on the enzymatical hydrolysis of chiral dichlorprop methyl ester
%A WEN Yue-zhong
%A ZHOU Shan-shan
%A FANG Zhao-hua
%A LIU Wei-ping
%A <br>WEN Yue-zhong
%A ZHOU Shan-shan
%A FANG Zhao-hu
%A LIU Wei-ping
%J 环境科学学报(英文版)
%D 2005
%I 
%X The effect of beta-cyclodextrins (beta-CDs) on the enzymatical hydrolysis of chiral dichlorprop methyl ester (DCPPM) was studied. Four kinds of beta-cyclodextrins (beta-cyclodextrin, Partly methylated-CD (PM-beta-CD), hydroxypropyl-cyclodextrin (HP-beta-CD) and carboxymethyl-cyclodextrin (CM-beta-CD)) were used. Compared with 100% DCPPM in the absence of betacyclodextrins, the activity of lipase decreased with the increase of Pbetacyclodextrin and PM-Pbetacyclodextrin. However, CM-beta-clodextrin stimulated the lipase activity. The inhibition effect of Pbeta cyclodextrin and PM-5betacyclodextrin on the hydrolysis of DCPPM is affected by many factors other than degree of the methylation blocking the active site of lipase. UV-Vis and Fourier transform infrared (FTIR) spectroscopy studies of the complexation of aqueous DCPPM with beta-CDs provide fresh insight into the molecular structure of the complex and explain the effects of beta-CDs on enzymatical hydrolysis of chiral DCPPM. Data showed that inclusion complexes had formed by complexation of the CM-beta-CD with DCPPM and the solubility of DCPPM was increased in water, which lead to the increased lipase activity.
%K cyclodextrins
%K enzyme
%K hydrolysis
%K chiral
%K dichlorprop<br>β-CD
%K CM
%K PM
%K HP
%K FTIR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=8BA6274B8F94FB88EEFC92B9DD18F5BC&yid=2DD7160C83D0ACED&vid=BCA2697F357F2001&iid=0B39A22176CE99FB&sid=FE4C96E058BB2280&eid=C812B90E96151014&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=0&reference_num=13