%0 Journal Article
%T Quantitation of metallothionein and cadmium in metallothionein in mink livers by anion-exchange HPLC-GFAAS method
%A Shan Xiaoquan
%A Sun Peng
%A Zheng Yan
%A Jin Longzhu Xu WeibingResearch Center for Eco-Environmental Sciences
%A Chinese Academy of Sciences
%A P O Box
%A Beijing
%A China
%A <br>Shan Xiaoquan
%A Sun Peng
%A Zheng Yan
%A Jin Longzhu
%A Xu Weibing
%J 环境科学学报(英文版)
%D 1992
%I 
%X Metallothionein (MT) has a great capacity of binding heavy metals showing an interesting connection with metal toxicology, as a biochemical marker for environmental metal pollution. Anino-exchange high per formance liquid chromatography (HPLC) was used to isolate and quantitate MT in livers of minks which were contaminated with heavy metals. MT isoforms (MT-I and MT-II) were eluted at approximately 11.3 and 14.3 min respectively from a DEAE-5 PW anion-exchange column with a Tris-HCl buffer (0.01 -0.25 mol/L, pH 8.6) and detected by UV absorbance at 254 nm. The cadmium concentrations in mink liver MT elutkms were determined by graphite furnace atomic absorption spectrometry (GFAAS) . Obvious increase in liver MT-I concentration rather than liver MT-II was found when the minks were contaminated by feeding contaminated fish captured from the heavy metal-polluted river. The cadmium concentration in mink liver MT-I also increased to some extent as the contaminated level increased.
%K metallothionein
%K cadmium
%K anion-exchange HPLC
%K GFAAS
%K heavy metal contaminated minks<br>metallothionein
%K cadmium
%K anion-exchange
%K HPLC
%K GFAAS
%K heavy
%K metal
%K contaminated
%K minks.
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=95E947898C849999CA6B7A2A3A68887C&yid=F53A2717BDB04D52&vid=E158A972A605785F&iid=CA4FD0336C81A37A&sid=9971A5E270697F23&eid=16D8618C6164A3ED&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=0&reference_num=0