%0 Journal Article
%T A method to extract algae toxin of microcystin-LR
%A ZHANG Ming-ming
%A PAN Gang
%A YAN Hai
%A CHEN Hao
%A <br>ZHANG Ming-ming
%A PAN Gang
%A YAN Hai
%A CHEN Hao
%J 环境科学学报(英文版)
%D 2004
%I 
%X A simple and low-cost method to obtain cyanobacterial toxin microcystin-LR(MC-LR) was developed. A new strain of Microcystis aeruginosa, named DC-1, producing microcystin-LR but not microcystin-RR, was separated from the field blooming algae samples of Dianchi Lake, in southwest of China. Following three times' freeze and thaw treatment, the cultivated DC-1 cells were extracted with 40% methanol in water. The extract was centrifuged and the supernatant applied to a Hydrophilic-Lipophilic Balance(HLB) SPE cartridge. Eluted impurities with a certain gradient from 30% to 50% methanol in water, MC-LR was finally eluted from the HLB cartridge with 60% methanol in water, and samples containing 3.85% to 14.8% of MC-LR were obtained. These MC-LR samples may be used in adsorption and biodegradation experiments instead of using expensive standard reagents.
%K purification
%K extraction
%K microcystins
%K HLB cartridge<br>海藻毒素
%K HLB
%K MC-LR
%K 环境污染
%K 细胞
%K 生态系统
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=05E552FDB54B28BB9F36EE464201208A&yid=D0E58B75BFD8E51C&vid=7801E6FC5AE9020C&iid=E158A972A605785F&sid=D02611D1F8166C9A&eid=A7379F6713A46835&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=3&reference_num=9