%0 Journal Article
%T Determnation of ochratoxin A in grain by monoclonal antibody-based enzyme-linked immunosorbent assay
%A Yang Chuanhe
%A Luo Xueyun
%A Liu Chang
%A Li Wenyan
%A Li Yiepeng
%A Zhao Danyu
%A Ji RongInstitute of Food Safety Control
%A inspection Ministry of Public HealthBeijing China
%A <br>Yang Chuanhe
%A Luo Xueyun
%A Liu Chang
%A Li Wenyan
%A Li Yiepeng
%A Zhao Danyu
%A Ji Rong
%J 环境科学学报(英文版)
%D 1994
%I 
%X The simple rapid and sensitive enzyme-linked immunosorbent assay (ELISA) methods, di-rect and indirect ELISA, for quantitation of ochratoxin A in cereal had been developed by theutilization of monoclonal antibody on immunomicroplate. Direct FLIAS was found to be less timeconsuming than indirect ELISA. For direct FLISA, recovery of 1 -500 ppb OA added to wheat was78.9-100.0% and rice was 88.9- 120.0%. For indirect EI.IAS, recovery of 1-500 ppb OA addedto wheat was 79.0- 110.0% and rice was 82.0 120.0%. The minimal detection level for OA was Ippb. Analyses of 31 samples that caused humanintoxicant for OA showed that the ELISA resultsagreed wtll with those obtained by thin-layer chromatogrdphy.
%K enzyme-linked immunosorbent assay (ELISA)
%K ochratoxin A
%K monoclonal antibody
%K cereal<br>enzyme-linked
%K immunosorbent
%K assay
%K (ELISA)
%K ochratoxin
%K A
%K monoclonal
%K antibody
%K cereal.
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=504CF1224357FDC87595AF627156BAB0&yid=3EBE383EEA0A6494&vid=B31275AF3241DB2D&iid=CA4FD0336C81A37A&sid=8477411EEDB08A86&eid=B62E0EEFE746E568&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=0&reference_num=0