%0 Journal Article
%T DNA-benzoquinone adducts analyzed by nuclease P1 m ediated 32P-postlabeling method
%A Jiang Xiangning
%A Liu Sufen
%A Xu Xiaobai
%J 环境科学学报(英文版)
%D 1992
%I 
%X With the super high sensitivity，high reproducibility and accumcy，nuclease P1 mediated 32P-postlabeling version has been successfully used to analyze the DNA-benzoquinone(DNA-BQ)adducts formed from m vitro cultured cells，reaction of benzoquinone ith calf thymus DNA and nucleoside monophosphates．It has been proven that the maior radioactive spot，contributing more than 70% of the total radioactivity of DNA-BQ adducts detected，is from deoxycytidine(dC)modified by benzoqumone while a minor one from deoxyguanosme(dGl．The method is capable of detecting 1 adduct in 109 to 1010 DNA bases．
%K P1
%K mediated
%K 32p-postlabelmg
%K DNA-BQ
%K adducts
%K dC-BQ
%K adduct
%K dG-BQ
%K adduct
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=AB754E631783FF067CF8C8B6D54DD7D4&yid=F53A2717BDB04D52&vid=E158A972A605785F&iid=E158A972A605785F&sid=C3BF5C58156BEDF0&eid=03F1579EF92A5A32&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=0&reference_num=0