%0 Journal Article
%T Isolation, identification and lysis properties analysis of a Vibrio parahaemolyticus phage VPp1<br>一株副溶血弧菌噬菌体VPp1 的分离鉴定及裂解性能
%A 彭 勇
%A 丁云娟
%A 林 洪
%A 王静雪
%J 海洋科学
%D 2013
%I 
%X In order to develop an effective bio-controlling method against Vibrio parahaemolyticus, a virulent phage VPp1 was isolated from aquatic sewage water and identified by plaque shapes, restriction, electronic microscope and lysis properties analyses. The results revealed that VPp1 contains a linear double stranded DNA with 15kb in size and it has an icosahedral head with 44 nm in diameter and does not have a tail, suggesting that VPp1 belongs to the Tectivirus family. VPp1 forms plaques with transparent center and large halo after culture in double-layer agar plate for 12 hours and its optimum MOI value was 0.0001. A one-step growth experiment showed that the latent period and burst size were estimated at 10 min and 90.3 phage particles/infected cell, respectively. VPp1 could be widely used because of its short latent period and large burst size.
%K phage
%K Vibrio parahaemolyticus
%K identification
%K lysis properties<br>噬菌体
%K 副溶血弧菌(Vibrio
%K )
%K 鉴定
%K 裂解性能
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=DFE7B94EB76B8F26135C9890832CEF6A&aid=AB3C2D65BF1EAC8E0AFA2D77C0AAC900&yid=FF7AA908D58E97FA&iid=CA4FD0336C81A37A&sid=6700D0D256586E73&eid=74011071555EB4E5&journal_id=1000-3096&journal_name=海洋科学&referenced_num=0&reference_num=0