%0 Journal Article
%T Molecular cloning and characterization of the key regulator of cell cycle cyclin B3 in Pacific Oyster (Crassostrea gigas), and its role in gonad development<br>长牡蛎细胞周期调控关键基因cyclin B3 的克隆及其在性腺发育中的作用
%A 王 桐
%A 李 莉
%A 阙华勇
%A 张国范
%J 海洋科学
%D 2011
%I 
%X The full length cDNA sequence of cyclin B3 was cloned from Pacific oyster (Crassostrea gigas) for the first time. Cyclin B3 cDNA was 2383 bp in length, containing a 1293bp CDS that encoded a peptide of 430 amino acids with a calculated molecular weight of 47.8 kD. Multiple alignment and converted domain analysis showed that this peptide was a homolog of cyclin B3. The gDNA sequence of cyclin B3 contained 10 exons and 9 introns, which was consistent with the position in evolution of C. gigas. Expression of cyclin B3 gene was found in all tissues, but also showed a very strong tissue-specific feature; the amount of cyclin B3 in gonad was the highest among tissues, 100 times of mantle, the lowest expression tissue. The mRNA amount of cyclin B3 in gonad increased with the maturation of gonad. The expression implied the important role of cyclin B3 in mitosis, meiosis and early development of embryo.
%K Pacific oyster (Crassostrea gigas)
%K cell cycle regulation
%K cyclin B3
%K gonad
%K Real Time PCR<br>长牡蛎(Crassostrea
%K )
%K 细胞周期调控
%K cyclin
%K B3
%K 性腺
%K Real
%K Time
%K PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=DFE7B94EB76B8F26135C9890832CEF6A&aid=76C08EEAB87DB3F806ECDE5C97377263&yid=9377ED8094509821&iid=59906B3B2830C2C5&sid=CA4FD0336C81A37A&eid=9CF7A0430CBB2DFD&journal_id=1000-3096&journal_name=海洋科学&referenced_num=0&reference_num=0