%0 Journal Article
%T Construction of high-guluronate-containing-alginate producing mutant of Pseudomonas sp. QDA<br>富含古罗糖醛酸的褐藻多糖生产菌株的构建
%A HU Bin
%A HAN Feng
%A LIN Yu-zi
%A GONG Qian-hong
%A YU Wen-gong
%A <br>胡斌
%A 韩峰
%A 林育姿
%A 宫倩红
%A 于文功
%J 海洋科学
%D 2008
%I 
%X Pseudomonas sp.QDA,which was isolated from seawater,has the ability of producing extracellular alginate.In order to obtain the high-guluronate-containing alginate,C-5-mannuronan epimerase gene(algG) of Pseudomonas sp.QDA was cloned by PCR and ligated in to plasmid pMF54Km.The resulting plasmid pMF54Km-algG was transferred to QDA by triparental mating,and an algG over-expressing strain,QDA-G,was obtained.The results of 1H-NMR showed that the M/G ratio of alginate fragments by QDA-G was 0.38 and the content of guluronate was 74.2%,about 26.4% higher than that of wild strain QDA.The genetic stability of QDA-G was good.No significant changes were found in M/G ratio of alginate fragments of QDA-G after 20 passages.
%K mannuronan C-5 epimerases
%K Pseudomonas sp
%K QDA
%K alginate
%K over-expression<br>甘露糖醛酸C-5差向异构酶
%K 假单胞菌
%K 褐藻多糖
%K 过量表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=DFE7B94EB76B8F26135C9890832CEF6A&aid=C58525519C59965EC99EA52B7397EC9C&yid=67289AFF6305E306&vid=9971A5E270697F23&iid=CA4FD0336C81A37A&sid=2A8D03AD8076A2E3&eid=EA389574707BDED3&journal_id=1000-3096&journal_name=海洋科学&referenced_num=0&reference_num=17