%0 Journal Article
%T Primary cell culture from gill explants of amphioxus<br>文昌鱼鳃组织原代培养
%A SHI Song-lin
%A HUANG Xiao-ming
%A LI Qi-fu
%A WANG San-ying
%A YANG Hai-bo
%A <br>石松林
%A 黄晓明
%A 李祺福
%A 王三英
%A 杨海波
%J 海洋科学
%D 2009
%I 
%X Primary cultures of gill cells were initiated from gill filament explants of amphioxus(Branchiostoma belcheri japonicum) by a tissue block culture method.The explants were cultured in Leibovitz L15 medium,M199,Eargle's MEM medium with 5%,10% or 20% foetal calf serum(FCS) and a certain amount of NaCl.We found that gill cell could grow better in 2 L15 medium than in others.The attachment efficiency was serum-dependent though increased FCS concentration did not stimulate further outgrowth of cells.The explants...
%K Branchiostoma belcheri japonicum
%K gill cell
%K primary cell culture<br>文昌鱼(Branchiostoma
%K belcheri
%K japonicum)
%K 鳃组织
%K 原代培养
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=DFE7B94EB76B8F26135C9890832CEF6A&aid=3A099D438481F4A1BCB99651AE409127&yid=DE12191FBD62783C&vid=27746BCEEE58E9DC&iid=38B194292C032A66&sid=1AE5323881A5ECDC&eid=B6DA1AC076E37400&journal_id=1000-3096&journal_name=海洋科学&referenced_num=0&reference_num=15