%0 Journal Article
%T Quality change of Pagrus major sperm after long-term cryopreservation<br>长期超低温保存后真鲷精子的质量变化
%A CHEN Ya-kun
%A LIU Qing-hua
%A ZHAO Chun-yan
%A XIAO Zhi-zhong
%A XU Shi-hong
%A Ma Dao-yuan
%A LI Jun
%A <br>陈亚坤
%A 刘清华
%A 赵春彦
%A 肖志忠
%A 徐世宏
%A 马道远
%A 李军
%J 海洋科学
%D 2010
%I 
%X After cryopreservation (1-73 months), the quality of Pagrus major sperm, which was cryopreserved from 2003 to 2009 in 5 batches, was examined by observing motility, fertilization rate and ultrastructures. The cryopreserved sperm were thawed in 40 oC water bath for 100~110 s. After activation with seawater, the motility of the sperm was estimated; 6-8 h after artificial fertilization, fertilization rate was examined; and after being fixed with by 2.5% glutaraldehyde, the ultrastructure of sperm was studied with scanning electron microscope and transmission electron microscope. The highest motility (87.67%±2.52%) and fertilization rate (71.33%± 8.84%) were obtained in sperm after a 1-month cryopreservation, even though they were not significantly different from those of the sperm after a 13-month or and 26-month cryopreservation (P>0.05). After a 48-month cryopreservation, the motility and fertilization rate decreased significantly in comparison with those of sperm after a 1-month cryopreservation (P<0.05). The lowest motility (50.67% ± 5.31%) and fertilization rate (39.56% ± 0.69%) were obtained in sperm cryopreserved for 73 months; main ultrastructural damage was observed in the head, mitochondria and membranes of sperm.
%K cryopreservation
%K Pagrus major
%K sperm
%K motility
%K fertilization rate<br>低温保存
%K 真鲷
%K (Pagrus
%K 精子
%K 运动率
%K 受精率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=DFE7B94EB76B8F26135C9890832CEF6A&aid=2171E2E74B77ACAEC1B0802A78365385&yid=140ECF96957D60B2&vid=339D79302DF62549&iid=B31275AF3241DB2D&sid=771152D1ADC1C0EB&eid=318E4CC20AED4940&journal_id=1000-3096&journal_name=海洋科学&referenced_num=0&reference_num=17