%0 Journal Article
%T Preparation and regeneration of protoplasts from Nannochloropsis oculata<br>微拟球藻原生质体制备与再生
%A MA Xiao-lei
%A ZHANG Lin
%A YANG Guan-pin
%A ZHU Bao-hua
%A PAN Ke-hou
%A <br>马晓磊
%A 张 琳
%A 杨官品
%A 朱葆华
%A 潘克厚
%J 海洋科学
%D 2012
%I 
%X The influence of enzyme digestion time and initial algal density on algal cells were considered.Protoplasts of Nannochloropsis oculata,a marine microalga,were prepareded by digestion with the mixture of 4% Hemicellulase and 2% Driselase.The protoplasts were examined after the treatment of Calcofluor White strain.The results shown that the highest preparation efficiency occurred at the initial cells density of 2×107~3×107 cells/mL and a digestion of 1～ 3h digestion.The resulting protoplasts could regenerate on regeneration medium.The growth of protoplasts was consistent with wild algal cells.Considered the influence of regeneration,we selected the optimal treatment time of 1h and initial algal density of 2×107 cells/mL.
%K Nannochloropsis oculata
%K protoplast preparation
%K regeneration
%K Calcofluor White staining<br>微拟球藻(Nannochloropsis
%K oculata)
%K 原生质体
%K 再生
%K Calcofluor
%K White
%K staining
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=DFE7B94EB76B8F26135C9890832CEF6A&aid=E2CD1BD993BDE604C80A502E4300092D&yid=99E9153A83D4CB11&vid=933658645952ED9F&iid=0B39A22176CE99FB&sid=2A8D03AD8076A2E3&eid=EA389574707BDED3&journal_id=1000-3096&journal_name=海洋科学&referenced_num=0&reference_num=0