%0 Journal Article
%T ULTRASTRUCTURAL STUDY ON PROTOPLASTS OF Porphyra haitanensis (BANGIOPHYCEAE, RHODOPHYTA)<br>坛紫菜原生质体的超微结构观察
%A 王素娟
%A 徐志东
%A 王光远
%A 夏镇澳
%J 海洋科学
%D 1986
%I 
%X The ultrastructure of protoplasts of Porphyra haitanensis (Bangiophyceae, Rhodophyta) is studied in this paper. Protoplasts of Porphyra were treated with enzyme mixture for two hours, followed by centrifugation. The enzyme mixture was composed of 1% sea snail digestive enzyme and 0.5% R-10 cellulase in 2 M glucose solution. Fluorescent microscope observation reveals that most of protoplasts obtained show no fluorescent reaction. The protoplasts were collected and fixed for the ultrastructural study. Observation under the electron microscope confirms that protoplasts have only one layer of plasma membrane surrounded without cell wall structure. The ultrastructure of protoplasts is similar to that of vegetative cells.
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=DFE7B94EB76B8F26135C9890832CEF6A&aid=7EE8650D5DAA3E8F&yid=4E65715CCF57055A&iid=E158A972A605785F&sid=659D3B06EBF534A7&eid=B91E8C6D6FE990DB&journal_id=1000-3096&journal_name=海洋科学&referenced_num=4&reference_num=0