%0 Journal Article
%T Enriched experiment and endogenous characteristics of polyphosphate-accumulating organisms(PAOs)<br>聚磷菌富集实验及其内源特征探究
%A WANG Qilin
%A HAO Xiaodi
%A CAO Yali
%A <br>王啟林
%A 郝晓地
%A 曹亚莉
%J 环境科学学报
%D 2010
%I 
%X By means of measuring maximal anaerobic phosphate release rates (PRR), analyzing 16S rRNA with fluorescence in-situ hybridization (FISH) and observing membrane integrity by live/dead staining, the aerobic decay characteristics of polyphosphate-accumulating organisms (PAOs) in a biological nutrient removal (BNR) system and an enriched PAOs sequencing batch reactor (SBR) system were investigated. It was experimentally determined that a highly enriched culture of PAOs (91%) was obtained in the enriched PAO-SBR system by alternating the carbon source in the feed between sodium acetate trihydrate and propionate over a 36 d switching cycle, i.e., sodium acetate trihydrate (24 d) and propionate (12 d). The experimental results and calculations revealed that the decay rates of PAOs in the BNR and enriched PAO-SBR systems were 0.113 d-1 and 0.181 d-1, respectively, and the death rates were 0.048 d-1 and 0.036 d-1. In addition, the results also demonstrated that cell death contributed 42% and 20%, respectively, to the cell decay in the BNR and enriched PAO-SBR systems. In other words, the activity decay accounted for 58% and 80% of the total cell decay. For this reason, cell death was not a major factor causing the cell decay of PAOs, but activity decay was mostly responsible for this process.
%K endogenous processes
%K cell decay
%K cell death
%K activity decay
%K phosphate release rate(PRR)
%K fluorescence in-situ hybridization(FISH)
%K LIVE/DEAD staining<br>内源过程
%K 细胞衰减
%K 细胞死亡
%K 活性衰减
%K 磷酸盐释放速率（PRR）
%K 荧光原位杂交（FISH）
%K LIVE/DEAD染色
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=03A55E61A8750ACAC6AF81EF9E2AC838&aid=634194EFEC069FC382E0826B64C73FC4&yid=140ECF96957D60B2&vid=340AC2BF8E7AB4FD&iid=59906B3B2830C2C5&sid=AAC7B2D4995BA8E4&eid=2F687D0B62DA15A6&journal_id=0253-2468&journal_name=环境科学学报&referenced_num=0&reference_num=0