%0 Journal Article
%T Characterization of phosphate removal by the psychrotolerant denitrifying bacterium Acinetobacter johnonii DBP-3 at low temperature<br>反硝化耐冷菌Acinetobacter johnonii DBP-3的低温除磷特性
%A LI Mingtang
%A HAO Linlin
%A LIU Mengyang
%A ZHAO Lanpo
%A <br>李明堂
%A 郝林琳
%A 刘梦洋
%A 赵兰坡
%J 环境科学学报
%D 2012
%I 
%X Under different conditions,the removal of phosphate by a psychrotolerant denitrifying bacterium Acinetobacter johnonii DBP-3(JN314436,CGMCC 4753) was studied by using batch testing experiment.Results showed that strain DBP-3 took up phosphate based on three types of electron acceptors in the order of oxygen > nitrate > nitrite.The capacity of stain DBP-3 in taking up phosphate ranged from 0 to 150 mg · L-1,and increased with increased nitrate concentration.On the other hand,phosphate removal rate obviously increased with the increased nitrite concentrations,which ranged from 0 to 20 mg · L-1;but there was no significant increase in the phosphate removal rate at nitrite concentration from 20 to 60 mg · L-1 and the rate of phosphate removal inversely decreased when the nitrite concentration increased over 80 mg · L-1.In anoxic condition,the removal rate of phosphate by cells of strain DBP-3 grew in cultural fluids with different sole carbon sources,following the order of sodium acetate > sodium citrate > glucose.The suitable range of pH value for strain DBP-3 in removing phosphate from cultural fluids was from 7 to 9.It should be noted that the strain DBP-3 removed phosphate even at 5 ℃ and the phosphate removal increased with the increased temperature from 5 to 30 ℃.However,the removal rate of phosphate decreased when the temperature increased to 35 ℃.Strain DBP-3 was more resistant to salinity in aerobic condition than in anoxic condition.The average phosphate content in cells of strain DBP-3 was up to 5.7%,after 48 h of anoxic incubation.In contrast,the lowest phosphate content in cells was 2.6% under anaerobic culturing condition.These showed that strain DBP-3 was one kind of phosphate accumulating /releasing microorganism.This study could provide theoretical basis for the biological treatment of eutrophic water body and nitrogen-and phosphate-rich sewage.
%K psychrotolerant bacteria
%K denitrifying phosphorus removal
%K electron acceptor
%K carbon source<br>耐冷菌
%K 反硝化除磷
%K 电子受体
%K 碳源
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=03A55E61A8750ACAC6AF81EF9E2AC838&aid=F6F80FAD71E363AE0B4F89791261AEF2&yid=99E9153A83D4CB11&vid=9971A5E270697F23&iid=DF92D298D3FF1E6E&sid=CDD86F65AA3A5D34&eid=3263523096AC9895&journal_id=0253-2468&journal_name=环境科学学报&referenced_num=0&reference_num=20