%0 Journal Article %T Using phospholipid fatty acid biomarkers for analysis of the microbial subcommunity in pigsty litter
利用磷脂脂肪酸生物标记分析猪舍基质垫层微生物亚群落的分化 %A ZHENG Xuefang %A LIU Bo %A LIN Yingzhi %A LAN Jianglin %A LIU Danying %A
郑雪芳 %A 刘波 %A 林营志 %A 蓝江林 %A 刘丹莹 %J 环境科学学报 %D 2009 %I %X The diversity of phospholipid fatty acid (PLFA) biomarkers was analyzed to investigate the microbial subcommunity in pigsty litter. The litter was treated with Luodong enzymes (Bacillus subtilis, provided by Fujian Luodong Biology Technology Co. Ltd) for 1 month, 6 months and 24 months. The results showed that PLFA biomarker distributions were significantly different among the three different treatments and in different layers (20, 40, 60, 80 cm depth) of the litter in the non-polluting pigsty. Some PLFA biomarkers were distributed in all layers of the litter system after all treatments, which is called complete distribution. However, some PLFA biomarkers were only distributed in limited layers of the litter system, called incomplete distribution. The distribution of individual PLFA biomarkers was also remarkably different in the litter layers in each treatment month. For example, the biomarker i17:0 3OH was distributed in the upper layers, but only minimally present in the 80 cm layer after 1 month of treatment, while it showed complete distribution in the 6 and 24 month treatments. In the cluster analysis, when the mahalanobis distance was 56.62, the PLFA biomarkers in the litter system of pigsty were grouped into three microbial subcommunities, named initial, transitional, and stable subcommunities. Analysis of the characteristics of each subcommunity showed that the initial subcommunity with 12.70 euclidean distance and transitional subcommunity with 71.10 euclidean distance could be divided into two groups containing different PLFA biomarkers, however, the stable subcommunity could be divided into three groups when the euclidean distance was 22.22. %K pigsty %K litter system %K subcommunity polarization %K phospholipid fatty acids
猪舍 %K 基质垫层 %K 亚群落分化 %K 磷脂脂肪酸(PLFA) %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=03A55E61A8750ACAC6AF81EF9E2AC838&aid=BEA99C1DB72B3FC7A48C57B86C534267&yid=DE12191FBD62783C&vid=771469D9D58C34FF&iid=708DD6B15D2464E8&sid=62430EC5AE2C2006&eid=A113722260CE9028&journal_id=0253-2468&journal_name=环境科学学报&referenced_num=0&reference_num=34