%0 Journal Article
%T Effect of cadmium on the expression of Fas and caspase-3 mRNA in chicken anterior pituitary<br>镉对鸡垂体Fas和caspase-3 mRNA表达的影响
%A HE Baoxi
%A FU Yequan
%A LI Jinlong
%A XU Shiwen
%A <br>何宝霞
%A 傅业全
%A 李金龙
%A 徐世文
%J 环境科学学报
%D 2008
%I 
%X The effect of cadmium chloride on cell apoptosis and the expressions of Fas and caspase-3 genes were explored in a hen model, Ninety normal Hyline white laying hens, aged 50 days, were randomly allocated to 3 groups of 30. The hens were fed 0 mg·kg-1 CdCl2 in the control group, 140 mg·kg-1 CdCl2 in the low-dosage cadmium group and 210 mg·kg-1CdCl2 in the high-dosage cadmium group. The anterior pituitary glands of the hens were removed after 20 d, 40 d and 60 d and the levels of cell apoptosis were detected by TUNEL and the expressions of Fas and caspase-3 genes were measured by RT-PCR. The results indicated that cadmium chloride can lead to apoptosis of pituicytes and overexpression of Fas and caspase-3 genes. At the same time point, cell apoptosis rates and gene expression showed variance (p<0.01) in both test groups compared with control and the low-dosage group changed greatly both the expression of Fas, caspase-3 mRNAand cell apoptosis rates. The data indicated that cadmium chloride could induce apoptosis of anterior pituitary cells. The expression of Fas and caspase-3 mRNAhave the same trend as apoptosis with the extension of time.
%K cadmium
%K anterior pituitary of chicken
%K apoptosis
%K Fas
%K caspase-3<br>镉
%K 鸡垂体
%K 凋亡
%K Fas
%K caspase-3
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=03A55E61A8750ACAC6AF81EF9E2AC838&aid=F6F579A6B2C02455E9E6A9D91605BB0B&yid=67289AFF6305E306&vid=D3E34374A0D77D7F&iid=DF92D298D3FF1E6E&sid=8EA44A8F6C7F424F&eid=AFD02B86BFB3C7FC&journal_id=0253-2468&journal_name=环境科学学报&referenced_num=2&reference_num=29