%0 Journal Article
%T Optimization of FISH to detect nitrite-oxidizing bacteria quantitatively<br>FISH技术定量解析亚硝酸盐氧化菌的条件优化
%A ZHANG Xing
%A LIN Weitie
%A ZHU Yanan College of bioscience
%A bioengineering
%A South China University of Technology
%A Guangzhou
%A <br>张星
%A 林炜铁
%A 朱雅楠
%J 环境科学学报
%D 2009
%I 
%X Experimental conditions of Fluorescence in Situ Hybridization(FISH)for detecting nitrite-oxidizing bacteria(NOB)are optimized through orthogonal tests,by using the common fluorescent probe based on the 16 S rRNA sequence of nitrite-oxidizing bacteria.Our results showed that the optimal conditions for sample preparation were a heat fixing-time of 2 hours,4% paraformaldehyde fixing-time of 15 minutes,and ethanol dehydration time of 5 minutes.The optimal conditions for detecting NOB consisted of a hybridizatio...
%K fluorescence in situ hybridization
%K nitrite-oxidizing bacteria
%K optimization<br>荧光原位杂交
%K 亚硝酸盐氧化菌
%K 优化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=03A55E61A8750ACAC6AF81EF9E2AC838&aid=56D83ED533130739AA2293D9CDBF69B7&yid=DE12191FBD62783C&vid=771469D9D58C34FF&iid=E158A972A605785F&sid=27350781F1397F32&eid=D7F3FD6D87AEF622&journal_id=0253-2468&journal_name=环境科学学报&referenced_num=1&reference_num=0