%0 Journal Article
%T Optimization of the fermentation medium for Pseudomonas strain producing a poly(vinyl alcohol)-degrading enzyme based on response surface methodology<br>聚乙烯醇降解酶产生菌发酵培养基响应面法优化研究
%A 王能强
%A 赵维俊
%A 梁芳
%A 李会东
%A 王栋
%J 环境科学学报
%D 2010
%I 
%X Two level factorial designs of the Plackett-Burman type were constructed to optimize the fermentation medium components of a poly(vinyl alcohol)-degrading enzyme produced by strain Pseudomonas sp. XT11-Z90S. The results showed that the concentrations of glucose, yeast extract and (NH4)2SO4 greatly influence the activity of poly(vinyl alcohol)-degrading enzyme. The optimum concentrations of glucose, yeast extract and (NH4)2SO4, determined by response surface methodology, were 0.64, 0.22 and 1.45 g·L-1, respectively. Under the optimal conditions mentioned above, the poly(vinyl alcohol)-degrading enzyme activity is 13.5U·mL-1, which was increased by 11.6% compared with the value before optimization.
%K poly(vinyl alcohol)-degrading enzyme
%K Plackett-Burman design
%K response surface methodology
%K optimization<br>聚乙烯醇降解酶
%K Plackett-Burman
%K design
%K 响应面
%K 优化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=03A55E61A8750ACAC6AF81EF9E2AC838&aid=915CF6425BF3891CF83D0C06224944F9&yid=140ECF96957D60B2&vid=340AC2BF8E7AB4FD&iid=B31275AF3241DB2D&sid=E2D70FDCD65737D1&eid=4E3541241257D204&journal_id=0253-2468&journal_name=环境科学学报&referenced_num=0&reference_num=13