%0 Journal Article
%T Optimized culture medium and fermentation conditions for enzyme production by the atrazine-degrading bacterium HB-5<br>莠去津降解菌HB-5的最佳产酶培养基及发酵条件
%A WANG Qian
%A ZHU Lusheng
%A WANG Jun
%A XIE Hui
%A LIU Wei
%A MA Tingting
%A DONG Xiaoli
%A <br>王倩
%A 朱鲁生
%A 王军
%A 谢慧
%A 刘伟
%A 马婷婷
%A 董晓丽
%J 环境科学学报
%D 2008
%I 
%X An atrazine degrading bacterium (Arthrobacter sp.) HB-5, was isolated in our laboratory from pesticide waste water. The optimum fermentation conditions and fermentation culture medium of HB-5 were selected by uniform experimental design and orthogonal experimentation, and the amount of enzyme produced by HB-5 was estimated from the atrazine degradation rate. The experimental results were analyzed by the SASsystem. The ideal composition of culture medium of HB-5 included saccharose 3.0 g·L-1, atrazine 0.38 g·L-1, K2HPO4 1.2 g·L-1, K2HPO4 0.5 g·L-1, MgSO4· 7H2O1.2 g·L-1, NaCl 0.1 g·L-1,microelements 3.8 mL·L-1. The optimum culture conditions of HB-5 were: culture time 48 h, inoculum size 2%, initial pH9.0 and amount of culture medium 80 mL. The degradation rate of atrazine by the optimum fermentation liquid reached up to 91.64%, and increased 125% over the original culture medium (40.67%).
%K atrazine
%K strain HB-5
%K fermentation
%K degradation rate<br>莠去津
%K 发酵
%K 细菌HB-5
%K 降解率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=03A55E61A8750ACAC6AF81EF9E2AC838&aid=6CE887D2A411B6B622EAAE4B7B86CE3C&yid=67289AFF6305E306&vid=D3E34374A0D77D7F&iid=0B39A22176CE99FB&sid=BF112261B65CB9C9&eid=8ED630AD8C61FAE8&journal_id=0253-2468&journal_name=环境科学学报&referenced_num=0&reference_num=13