%0 Journal Article
%T Genetic diversity of microbial communities in farmland soils contaminated with mixed heavy metals<br>重金属复合污染农田土壤的微生物群落遗传多样性研究
%A ZHAO Xiangwei
%A LUO Yongming
%A TENG Ying
%A LI Zhengao
%A SONG Jing
%A WU Longhua
%A <br>赵祥伟
%A 骆永明
%A 滕应
%A 李振高
%A 宋静
%A 吴龙华
%J 环境科学学报
%D 2005
%I 
%X Four soil samples contaminated with the different levels of mixed heavy metals were collected from a nearby smelter and the soil DNA was extracted by FastPrep system from which fragments were amplified. The genetic diversity of the soil microbial communities was analyzed by DGGE (denaturing gradient gel electrophoresis) of a conservative region of 16S rRNA gene. The results indicated that the mixed heavy metal pollution affected soil microbial community diversity, but there were not the simply negative relations between mixed heavy metal contamination and genetic diversity of soil microbial communities, and the largest diversity indexes appeared in the moderate mixed polluted soil. The reason might be that heavy metals changed the competitive relationships among individuals of soil microbial community, which lead to the lose of the dominance of the original predominant populations, or that the bacteria resistant to heavy metals protected other more sensitive ones.
%K mixed heavy metal pollution
%K soil microbial community
%K 16S rDNA
%K DGGE<br>农田土壤
%K 遗传多样性
%K 微生物群落
%K 总DNA
%K 多样性指数
%K 采集
%K 变性梯度凝胶电泳
%K 重金属复合污染
%K 污染程度
%K 冶炼厂
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=03A55E61A8750ACAC6AF81EF9E2AC838&aid=0A34EC44E3F09B76&yid=2DD7160C83D0ACED&vid=C5154311167311FE&iid=0B39A22176CE99FB&sid=50BBDFAC8381694B&eid=AC1578C6BB9EBDEF&journal_id=0253-2468&journal_name=环境科学学报&referenced_num=32&reference_num=28