%0 Journal Article %T Effect of m-cresol on the growth of coriolus versicolor and its laccase product ion
间甲酚对杂色云芝(Coriolus versicolor)生长及产漆酶能力的影响 %A 吴坤 %A 徐淑霞 %A 闵航 %A 吕镇梅 %A 汪伦记 %A 陈红歌 %A 喻子牛 %J 环境科学学报 %D 2004 %I %X A systematic research of m-cresol biodegradation dynamics by Corio lus versicolor was made. The fermentation characters by C. versicolor in 30 L fermen tor was performed and DC(dried cell), starch, m-cresol consumption, and lac case activity was determined with an 12h interval in three cases of 0mg·L-1, 166.7m g·L-1 and 275mg·L-1 m-cresol. In the absence of the m-cresol, the maximum densi ty of DC was 2600mg·L-1 at 188h, the enzyme activity appeared at 120h, reached the maximum of 82.15U/mL at 228h and then gradually remained stably during the l ater contabescence periods. In the presence of 166.7mg·L-1m-cres ol,, the maxim um DC was 995mg·L-1 at 204h, which was much lower (reduced to 61.73% of nom-cresol) than that in the absence of m-cresol, but the enzyme activity appeare d at 180h, the maximum was 118.36U·mL-1 at 204h, 44.1%higher than tha t in the abse nce of m-cresol, and then was stable without obvious variation in the late r sta ge. In the presence of 275mg·L-1 m-cresol, the maximum DC was 32 0mg·L-1 at 37 2h, which was 12.31% of the absence of m-cresol and 32.16% of the prese nce of 1 66.7mg·L-1 m-cresol. The enzyme activity appeared at 264h, and the maximum one w as112.8U·mL-1 at 396h, which was similar to that at the 166.7mg· L-1 m-cresol co ncentration, indicating that m-cresol could reduce the higher productivity of C. versicolor for laccase. The time-order model of Coriolus versicolor grow th at t hree concentrations of m-cresol above was studied and the kinetics equation s of the growth model were simulated with one order Logistic Equation. Thus, the addi tion of m-cresol doesn't change the growth kinetics model of Coriolus ver sicolor . The kinetics of the starch consumption could be described by a Logistic model, respectively in the above three cases, which indicated that m-cresol coul d, in part, replace or inhibit the consumption of the starch. The degradation kinetic s of m-cresol by C. versicolor in the fermentative liquid was also disc ussed. At m-cresol concentration of 275mg·L-1, its degradation appeared wit h an unstabl e and slow degradation inclination. The different equations also showed that the degradation kinetics of m-cresol by C. versicolor was different at var ious concentrations. %K white-rot fugus %K coriolus versicolor %K pollutant %K m-cresol %K b %K iodegradation %K dynamics model %K kinetics equation
间甲酚 %K 消耗 %K 淀粉 %K 代替 %K 酶活 %K 漆酶 %K 稳定态 %K 干重 %K 浓度 %K 菌丝 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=03A55E61A8750ACAC6AF81EF9E2AC838&aid=AC65099C7378EC11&yid=D0E58B75BFD8E51C&vid=B91E8C6D6FE990DB&iid=B31275AF3241DB2D&sid=4A8E5D086592D016&eid=39B73ADA6F3DD05F&journal_id=0253-2468&journal_name=环境科学学报&referenced_num=0&reference_num=16