%0 Journal Article
%T Study on the extraction and purification of microcystins<br>微囊藻毒素的提取和提纯研究
%A YAN Hai
%A PAN Gang
%A ZHANG Mingming
%A CHEN Hailiu
%A ZOU Hua
%A <br>闫海
%A 潘纲
%A 张明明
%A 陈海柳
%A 邹华
%J 环境科学学报
%D 2004
%I 
%X The methods for extracting and purifying microcystin (MCs) in dry cyanobacterial cells taken from Dianchi Lake, Yunnan Province, were studied in this paper. 40% methanol-water solution was found to be an optimum solution for effectively extracting MC-RR and MC-LR from cyanobacterial cells among different ratios of methanol when the concentration of dry cyanobacterial cells was 20g·L-1. After the extraction solution of 40% methanol containing MCs was passed through a cartridge (Waters, OASIS~(TM) HLB Cartridge), MCs were purified by the elution with 70% methanol, and 7.3% and 3.5% purities of MC-RR and MC-LR were obtained. By collecting the achromatic fraction of the elution after blue-green and orange-yellow color elution, MC-RR and MC-LR with the purities of 28.6% and 12.9% were obtained.
%K microcystins
%K extraction
%K purification<br>微囊藻毒素
%K 提取
%K 提纯
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=03A55E61A8750ACAC6AF81EF9E2AC838&aid=605CE9A3A8E44699&yid=D0E58B75BFD8E51C&vid=B91E8C6D6FE990DB&iid=0B39A22176CE99FB&sid=26AEEED215BE97D0&eid=3356A7630A93A219&journal_id=0253-2468&journal_name=环境科学学报&referenced_num=11&reference_num=19