%0 Journal Article
%T Construction of VR1+cell model and verification of Nielsen''s hypothesis<br>VR1+细胞模型的构建和Nielsen假说的验证
%A YAN Yan
%A QIAO Yongkang
%A XU Qian
%A WANG Li
%A DING Shumao
%A GUO Jianjun
%A ZHOU Yanqing
%A LIU Dandan
%A YANG Xu
%A <br>严彦
%A 乔永康
%A 徐钱
%A 王丽
%A 丁书茂
%A 郭建军
%A 周砚青
%A 刘丹丹
%A 杨旭
%J 环境科学学报
%D 2006
%I 
%X To explore the physiological role of vanilloid receptor type 1 (VR1) as a possible bio-target molecule of indoor-air pollutant of formaldehyde, VR1 -HEK293 cell model was constructed using the VR1-cDNA plasmid through the molecular cloning and transfection technology. The experiments of capsaicin and formaldehyde inducing death of transfected VR1 -HEK293 cells were carried out. The results showed that VR1 -HEK293 cell model was constructed successfully, and measurement of VR1 -HEK293 cell death rate showed to be an available and effective method, which could be applied to detecting the VR1 expression in cells in vitro. And activated VR1 by formaldehyde could be blocked by capsazepine, a specific antagonist of VR1, Therefore it was suggested that the irritant effect of airway might be also mediated by VR1. Therefore, it was concluded that VR1 as a possible bio-target molecule of formaldehyde, and Nielsen's hypothesis was correct according to our experiments in vitro.
%K formaldehyde
%K vanilloid receptor
%K VR1 -HEK293 cell model
%K Nielsen's hypothesis<br>甲醛
%K 类香草素受体
%K VR1
%K -HEK293细胞模型
%K NIelsen假说
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=03A55E61A8750ACAC6AF81EF9E2AC838&aid=C029DDDC4B01E31C&yid=37904DC365DD7266&vid=96C778EE049EE47D&iid=CA4FD0336C81A37A&sid=A020552C37306588&eid=2DBBF45CC176713E&journal_id=0253-2468&journal_name=环境科学学报&referenced_num=0&reference_num=17