%0 Journal Article
%T Effect of Immobilization on Biosensor for Benzene Derivates Detection<br>固定化条件对苯系物细胞传感器检测效果的影响
%A TANG Kuo
%A MA An-zhou
%A YU Qing
%A DENG Xue-mei
%A LV Di
%A ZHUANG Guo-qiang
%A <br>唐阔
%A 马安周
%A 于清
%A 邓雪梅
%A 吕迪
%A 庄国强
%J 环境科学
%D 2013
%I 
%X A whole cell sensor, Pseudomonas fluorescens A506(pTS), was immobilized by sodium alginate and the factors of cell density, immobilization time and beads usage were optimized. The performance of the immobilized cells was compared with that of the free cells. After 2 h immobilization,the increasing speed of fluorescent signal of immobilized cells was 2.26 times as high as that of the free cells,and the peak value was 2.23 times as high during the detection time ranging from 1.5 to 6.0 h. The constantly lower growth and density of the immobilized cell implied the enhanced signal intensity of single cells after immobilization. Meanwhile, the cell density decreased as the immobilization time prolonged. Cell density and immobilization time were the dominant factors affecting the detection signal. For benzene at higher concentrations, the immobilized biosensor showed more rapid signal response at the early period of detection.
%K benzene derivates detection
%K immobilized cell
%K cell density
%K immobilization time
%K beads usage<br>苯系物检测
%K 固定化细胞
%K 细胞浓度
%K 固定化时间
%K 投加量
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=9C65539B20E123FC868FC406B5086074&yid=FF7AA908D58E97FA&vid=339D79302DF62549&iid=0B39A22176CE99FB&sid=8D71AF42ACD39979&eid=34A7AB0452E6AF23&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=28