%0 Journal Article
%T Construction of Electrochemiluminescence System for Harmful Algae Detection<br>赤潮藻电致化学发光分子探针检测系统的构建
%A ZHU Xi
%A ZHEN Yu
%A MI Tie-zhu
%A CHI Zhen-ming
%A XU Xiao-chun
%A <br>朱霞
%A 甄毓
%A 米铁柱
%A 池振明
%A 徐晓春
%J 环境科学
%D 2012
%I 
%X To develop a new technique, Electrochemiluminescence-Molecular Probe (ECL-MP), for harmful algae detection, a highly sensitive electrochemiluminescence (ECL) detection system was set up based on the principle of ECL and related literature. The optimization tests were carried out, including the impact of voltage and current, the concentration of tripropylamine (TPrA) and the pH of phosphate buffer (PBS). The determination limit of Ru(bpy)3Cl2·6H2O was 10-11 mol·L-1 and the detection range was from 10-9-10-5 mol·L-1as well as the detection amount of substance was in the range from 0.4 pmol-4 nmol in the optimal reaction conditions with voltage of 1.0 V, current of 1.0 mA, TPrA concentration of 1.5 mol·L-1 and pH of 7.4. It was proved by facts that this ECL analyzer was stable and highly sensitive, which was helpful in establishment of ECL-MP.
%K 赤潮藻
%K 电致化学发光
%K 光信号
%K 联吡啶钌
%K 三丙胺
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=4189763B19B8AD454A832B3D33ABD9E6&yid=99E9153A83D4CB11&vid=27746BCEEE58E9DC&iid=CA4FD0336C81A37A&sid=8B59EA573021D671&eid=0DEB7A8A66C33AAD&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=27