%0 Journal Article
%T Study on Structural Characterization and Purification of Bioflocculant ZS-7<br>微生物絮凝剂ZS-7的纯化及其结构特征研究
%A LI Zhong
%A ZHANG Xiu-yu
%A QIN Jian-qiao
%A WANG Kai-feng
%A <br>黎忠
%A 张修玉
%A 秦建桥
%A 王开峰
%J 环境科学
%D 2010
%I 
%X The bioflocculant ZS-7 was purified to homogeneity by ethanol precipitation, dialysis and gel permeation chromatography (GPC). About 0.98 g of the purified bioflocculant could be recovered from 1L of fermentation broth. The purified bioflocculant was identified as a glycoprotein consisting of polysaccharide (91.5%) and protein (8.4%), with an approximate molecular weight of 6.89 x 10(4). The major component of ZS-7 is an acid polysaccharide including uronic (16.4%), pyruvic (7.1%) and acetic acids (0.5%). It consists of galactose, glucose, mannose and rhamnose in a molar ratio of 142 :2.2 : 4.5 : 3.4. X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) and NMR spectrum of the bioflocculant indicate the presence of carboxyl, hydroxyl, amide, amino, methoxyl and sulfate groups.
%K bioflocculant
%K Bacillus licheniformis X14
%K glycoprotein
%K purification
%K structural characterization<br>微生物絮凝剂
%K 地衣芽孢杆菌X14
%K 糖蛋白
%K 纯化
%K 结构特征
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=CDD67C9345DF1E5EAFBD6F353F74C612&yid=140ECF96957D60B2&vid=4AD960B5AD2D111A&iid=9CF7A0430CBB2DFD&sid=2388B0C36A6A2DBD&eid=FFE181911AE93FD6&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=0