%0 Journal Article
%T Effect of Cellulose-decomposing Strain on Microbial Community of Cow Manure Compost<br>接种纤维素降解菌对牛粪堆肥微生物群落的影响
%A LIU Ji
%A LI Wan
%A XU Xiu-hong
%A LI Hong-tao
%A <br>刘佳
%A 李婉
%A 许修宏
%A 李洪涛
%J 环境科学
%D 2011
%I 
%X Taking the cow dung and straw as composting raw materials, effect of cellulose-decomposing strain on microbial community of cow manure compost was investigated with the traditional culture method and PCR-DGGE technique. The results showed that the microbiological inocula showed a more rapid rate of temperature elevation at the start of composting and prolonged the time of high-temperature process and increased the number of microbial. The DGGE map of cellulose-decomposing strain compost was different from natural compost, the succession of microbial community in cellulose-decomposing strain was faster than natural compost. Sequence comparison revealed that the Pseudomonassp.of bacterial appeared at the initial stage and Acinetobacter sp.,Flavobacteria were existed at the high-temperature process in natural compost; while Arthrobacter sp. was appeared at the high-temperature process in cellulose-decomposing strain compost. Bacillus sp. was dominant species at middle and later stage in natural compost and cellulose-decomposing strain compost. Eimeriidae of fungal appeared in compost materials, Aspergillus and thermophilic fungi were dominant species at the high-temperature process in natural compost and cellulose-decomposing strain compost. Ascomycota appeared at middle and later stage in natural compost; while Basidiomycetes in cellulose-decomposing strain compost. Aspergillus was found throughout the process.This result suggested that the microbiological inocula were able to facilitate the bacterial microbial diversity of the compost; reduced the fungal microbial diversity of the compost. The aims of this study were to provide a scientific basis to the diversity of microbial community by monitoring the dynamics of microbial community in cellulose-decomposing strain compost and represent an important step towards the understanding of microbiological inocula and its function in the degradation process of compost.
%K cow dung
%K compost
%K microbial community
%K cellulose-decomposing strain
%K denaturing gradient gel electrophoresis (DGGE)<br>牛粪
%K 堆肥
%K 微生物群落
%K 纤维素降解菌
%K 变性梯度凝胶电泳(DGGE)
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=D1920AB56F28E37CBD081CFF32FE8908&yid=9377ED8094509821&vid=9971A5E270697F23&iid=F3090AE9B60B7ED1&sid=257D0C1BEDE64186&eid=EFBD42109B48A699&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=26