%0 Journal Article
%T Effect of Biofilm on Nanoscale Zero-Valent Iron-Microorganism Removing NO-3-N in Groundwater<br>生物膜对纳米铁-微生物去除地下水中NO-3-N的影响
%A LI Ning
%A JIN Zhao-hui
%A LI Tie-long
%A XIA Hong-cai
%A ZHANG Na
%A <br>李宁
%A 金朝晖
%A 李铁龙
%A 夏宏彩
%A 张娜
%J 环境科学
%D 2011
%I 
%X Nanoscale zero-valent iron (NZVI) was synthesized by reduction in liquid phase, and autotrophic denitrifying bacteria were domesticated from activated sludge by self-made chemostat. Compared the denitrifying effects of adding NZVI only with that of adding booth NZVI and bacteria in nitrate culture fluid, including investigation of three forms of nitrogen(NO-3-N,NO-2-N,NH3/NH+4-N) by spectrophotometer, and investigating the variety of pH in nitrate culture fluid by pH meter. Combining acridine orange (AO) dyed fluorescence photomicrograph, proved that biofilm has a leading role both in corrosion process of NZVI and in process of biotic denitrification of NZVI-bacteria system. Based on this, compared denitrifying effect in the circumstance of adding NZVI and bacteria separately with that in the circumstance of adding biofilm-NZVI composite which was made with glucose providing nutrition. Result shows that in the circumstance of adding NZVI and bacteria separately, NO-3-N in the system can be removed completely within 10 days, there are 37% of NO-3-N was transformed into ammoniacal nitrogen, the rest 63% of NO-3-N should have left solution in the form of gaseous denitrifying products. In the circumstance of adding biofilm-NZVI composite, NO-3-N in the system can be removed completely within 5 days, there are 28% of NO-3-N be transformed into ammoniacal nitrogen, the rest 72% of NO-3-N should have left solution in the form of gaseous denitrifying products. Experimental result also shows biofilm-NZVI composite has a greater abundance of microorganism and is more active in denitrification, it is suitable for removing the nitrate pollutant in groundwater.
%K 生物膜
%K 纳米零价铁
%K 硝酸盐污染
%K 地下水
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=132EE59BB32962AB2E261A5379D85456&yid=9377ED8094509821&vid=9971A5E270697F23&iid=B31275AF3241DB2D&sid=28132669ED57E050&eid=E8EF49FD27992E02&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=28