%0 Journal Article
%T Development of Direct Competitive Enzyme-Linked Immunosorbent Assay for the Determination of Domoic Acid<br>软骨藻酸直接竞争ELISA方法的建立及优化
%A WANG Qian
%A CHENG Jin-ping
%A GAO Li-li
%A DONG Yu
%A XI Lei
%A <br>王茜
%A 程金平
%A 高利利
%A 董宇
%A 席磊
%J 环境科学
%D 2012
%I 
%X To develop a direct competitive enzyme-linked immunosorbent assay (ELISA) for rapid detection of domoic acid concentrations, HRP (horse radish peroxidase) was successfully linked to DA using EDC. The concentration of DA was quantitatively analyzed on the basic of the specific immune responses between the DA- HRP and the monoclonal antibodies made in advance. Calibration curve were established after the optimization of reaction conditions such as the type of blocking solution, the blocking time and the incubation temperature. The results show that, the best reaction condition of the direct competitive ELISA is 1% gelatin, blocking 1 h at 37 degrees C, incubating 1 h at 37 degrees C after the monoclonal antibodies added. The detect limit is 3.58 ng x mL(-1), the coefficient of variation between the holes is below 15%, and the recovery is 80% - 120%. The whole analysis process could be completed within 1.5 h. It meets the requirements of rapid and batch detection of domoic acid. The method will have broad development prospects.
%K domoic acid(DA)
%K enzyme-linked immunosorbent assay(ELISA)
%K antibody
%K antigen
%K rapid detection<br>软骨藻酸(DA)
%K 酶联免疫吸附法(ELISA)
%K 抗体
%K 抗原
%K 快速检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=2196949E2245EB60C9CBC540366674C9&yid=99E9153A83D4CB11&vid=27746BCEEE58E9DC&iid=0B39A22176CE99FB&sid=AEE2F90BC0DB5F35&eid=7004BE6E41AAF52C&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=25