%0 Journal Article
%T Diversity of Culturable Butane-oxidizing Bacteria in Oil and Gas Field Soil<br>油气田土壤样品中可培养丁烷氧化菌多样性研究
%A ZHANG Ying
%A LI Bao-zhen
%A YANG Jin-shui
%A WANG Shuang-qing
%A YUAN Hong-li
%A <br>张莹
%A 李宝珍
%A 杨金水
%A 汪双清
%A 袁红莉
%J 环境科学
%D 2012
%I 
%X Butane-oxidizing bacteria in soil sample sites from Puguang gas field in Sichuan province and Jianghan oil field in Hubei province were isolated and 16S rRNA gene sequence and phylogenetic analysis were applied. The differences of number and phylogenetic position and population diversity of hydrocarbon-oxidizing bacteria in different environment were investigated. The results show that 25 strains of butane-oxidizing bacteria were isolated. Based on sequencing of 16S rRNA gene, the species of bacteria in two samples are classified into 3 phyla including Firmicutes, Actinobacteria and Proteobacteria. The community structure of butane-oxidizing bacteria isolated from two oil samples is simple, both of them contain 4 genus including Bacillus, Pseudomonas, Rhodococcus and Arthrobacter. Strains in the genus of Ochrobactrum and Mycobacterium were only isolated from Puguang gas field. The number and population diversity of butane-oxidizing bacteria in Puguang gas field was more than those in Jianghan oil field.
%K butane-oxidizing bacteria
%K microbial prospect oil and gas
%K 16S rRNA gene
%K butane
%K phylogenetic analysis<br>丁烷氧化菌
%K 微生物油气勘探
%K 16S
%K rRNA基因
%K 丁烷
%K 系统发育分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=4189763B19B8AD45F85B58FAA17D4CD7&yid=99E9153A83D4CB11&vid=27746BCEEE58E9DC&iid=CA4FD0336C81A37A&sid=BF112261B65CB9C9&eid=8ED630AD8C61FAE8&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=23