%0 Journal Article
%T Detecting the Cytotoxicities of Five Bisphenol A Analogues to the MCF-7 Human Breast Carcinoma Cell Line Through Different Endpoints<br>不同终点检测5种双酚A类化合物对MCF-7的细胞毒性
%A ZHANG Shuai-shuai
%A LIU Yan
%A LIU Shu-shen
%A ZHU Xiang-wei
%A <br>张帅帅
%A 刘堰
%A 刘树深
%A 朱祥伟
%J 环境科学
%D 2012
%I 
%X As the main synthetic raw materials of polycarbonate, bisphenol A (BPA) and its analogues have been important issues in environmental pollution. The current studies focus mainly on BPA's estrogen effects and little on their cytotoxic effects. To assess the cytotoxicities of the five BPA analogues, we employed the MTS assay to determine the inhibition toxicity to MCF-7 (ER-), 2,4-dinitrophenylhydrazine assay to determine the release rate of lactate dehydrogenase (LDH) escaping into cell culture medium, and single cell gel electrophoresis assay (SCGE) to detect DNA damage. The dose-response curves (DRC) between the observed inhibition toxicities and concentrations of the BPA compounds in MTS assay were fitted by using the nonlinear least squares (NLS) and the results showed that all the dose-response relationships were effectively described by the Weibull or Logit function. The toxicities expressed by -lgpEC50 were BPB>BPC>TDP>BPE>BPA. LDH assay and SCGE assay showed that when the concentrations of BPA analogues were EC20, the MCF-7 cell proliferation was slightly inhibited due to its little damaged DNA, and at EC40the cell proliferations were significantly inhibited due to the seriously damaged DNA, leading to the damage of cell membrane and release of LDH.
%K BPA
%K MCF-7
%K cytotoxicity
%K SCGE
%K LDH<br>双酚A(BPA)
%K MCF-7
%K 细胞毒性
%K 单细胞凝胶电泳(SCGE)
%K 乳酸脱氢酶(LDH)
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=DEBC7BFAFBD89922A2F58C4DFAD9B7D8&yid=99E9153A83D4CB11&vid=27746BCEEE58E9DC&iid=708DD6B15D2464E8&sid=C255FB60BAD7A2D1&eid=25EE41C74F3A8BD7&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=28