%0 Journal Article
%T Detection of Prorocentrum minimum (Pavillard) Schiller with the Electrochemiluminescence-Molecular Probe<br>应用电致化学发光分子探针技术对微小原甲藻的检测
%A ZHU Xi
%A ZHEN Yu
%A MI Tie-zhu
%A YU Zhi-gang
%A CHI Zhen-ming
%A LU Xing-lan
%A <br>朱霞
%A 甄毓
%A 米铁柱
%A 于志刚
%A 池振明
%A 路兴岚
%J 环境科学
%D 2012
%I 
%X In this study, the electrochemiluminescence-molecular probe (ECL-MP) was established based on the sandwich hybridization integrated with nuclease protection assay (NPA-SH) improved by electrochemiluminescence (ECL). It can be used for detecting Prorocentrum minimum (Pavillard) Schiller qualitatively and quantitatively. NPA probes of P. minimum were designed based on the NPA-SH. After labeled with Ru (bpy)3(2+) and biotin, they bounded with streptavidin-coupled magnetic beads and generated electrochemiluminescence in an ECL analyzer. The ECL counts per second (CPS) was drawn against the cell number obtained from microscopy to establish a calibration curve of P. minimum. The results showed that the labeled NPA probes had good specificity and practicability, the optimal usage of magnetic beads was 4 microg for detecting 20 microL hybridization mixture, the detection range of P. minimum cell numbers was 6.25 x 10(2) - 4 x 10(4), and there was no significant difference between the data gained from ECL-MP and microscopy with 95% confidence level (t-test) when treated with cultured and mixed samples. ECL-MP was a convenient new tool for rapid assessment of P. minimum in marine environment.
%K electrochemiluminescence(ECL)
%K sandwich hybridization integrated with nuclease protection assay(NPA-SH)
%K Prorocentrum minimum(Pavillard) Schiller
%K Ru(bpy)3Cl2·6H2O
%K tripropylamine(TPrA)<br>电致化学发光
%K 双特异分子探针
%K 微小原甲藻
%K 联吡啶钌
%K 三丙胺
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=2196949E2245EB6048E7798E8D116D9C&yid=99E9153A83D4CB11&vid=27746BCEEE58E9DC&iid=0B39A22176CE99FB&sid=DB7B2C790D19BE6E&eid=6C62BFE34266FA92&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=28