%0 Journal Article
%T Phylogenetic Analysis of Methanogenic Corn Stalk Degrading Microbial Communities<br>玉米秸秆厌氧降解复合菌系的微生物群落结构
%A QIAO Jiang-tao
%A GUO Rong-bo
%A YUAN Xian-zheng
%A SHI Xiao-shuang
%A XU Xiao-hui
%A FAN Xiao-lei
%A QIU Yan-ling
%A <br>乔江涛
%A 郭荣波
%A 袁宪正
%A 师晓爽
%A 许晓晖
%A 范晓蕾
%A 邱艳玲
%J 环境科学
%D 2013
%I 
%X Methanogenic corn stalk degrading enrichment cultures were constructed using corn stalk as the sole carbon source and eight types of environmental samples as inocula. All the cultures could degrade corn stalk within 30-50 days and the total solids (TS) removal rates were in the range of 30%-40%. In six out of eight cultures, the cumulative methane yields per gram TS were 62.1-118.4 mL·g-1, with acetate, propionate and butyrate as the major volatile fatty acids (100-500 mg·L-1), and the final pH were 6.5-6.7. In the other two cultures, the cumulative methane yields per gram TS were 8.5-9.7 mL·g-1, while the concentrations of acetate were high (1200 mg·L-1), and the final pH were low (5.6-5.9). The bacterial and archaeal structures in eight enrichments were investigated with a 16S rRNA genes-based clone library method. Clones belonging to the bacterial phyla Firmicutes, Bacteroidetes, Synergistetes and Thermotogae were observed in abundance within the bacterial clone libraries, which accounted for 37.8%, 34.3%, 11.6% and 6.4% of the total number of bacterial clones, respectively. Within the domain Archaea, clones affiliated with the classes Methanomicrobia and Methanobacteria were found to be abundant in the archaeal clone libraries, which accounted for 61.1% and 38.9% of the total number of archaeal clones, respectively.
%K corn stalk
%K anaerobic degradation
%K methane
%K microbial community
%K 16S rRNA gene clone library<br>玉米秸秆
%K 厌氧降解
%K 甲烷
%K 群落结构
%K 16S
%K rRNA
%K 基因克隆文库
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=FA4CA184ED44CF9182DAD2CFB1075992&yid=FF7AA908D58E97FA&vid=339D79302DF62549&iid=E158A972A605785F&sid=F41021172F25CDE0&eid=B08191F41006DCF9&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=29