%0 Journal Article
%T Isolation of a Monocrotophos-Degrading Bacterial Strain and Characterization of Enzymatic Degradation<br>久效磷降解菌的分离及其酶促降解特性研究
%A JIA Kai-zhi
%A LI Xiao-hui
%A HE Jian
%A GU Li-feng
%A MA Ji-ping
%A LI Shun-peng
%A <br>贾开志
%A 李晓慧
%A 何健
%A 顾立锋
%A 马吉平
%A 李顺鹏
%J 环境科学
%D 2007
%I 
%X A monocrotophos dimethyl (E)-1-2-methylcarbamoylvinylphosphate or MCP] -degrading strain named as M-1 was isolated from sludge collected from the wastewater treatment pool of a pesticide factory and identified as Paracoccus sp. according to its morphology and biochemical properties and 16S rDNA sequence analysis. Using MCP as a sole carbon source, M-1 was able to degrade MCP(100 mg x L(-1)) by 92.47% in 24 h. The key enzyme(s) involved in the initial biodegradation of monocrotophos in M-1 was shown to be constitutively expressed cytosolic proteins and showed the greatest activity at pH 8.0 and 25 degrees C, with its Michaelis-Mentn's constant (K(m)) and maximum degradation rate (V(max)) of 0.29 micromol x mL(-1) and 682.12 micromol (min x mg)(-1) respectively. This degrading enzyme(s) was sensitive to high temperature, but kept high activity under alkaline conditions.
%K monocrotophos
%K biodegradation
%K Paracoccus sp
%K enzymatic degradation<br>久效磷
%K 生物降解
%K Paracoccus
%K sp.
%K 酶促降解
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=A38D5E81E3CA88A50A2A365F7830C47D&yid=A732AF04DDA03BB3&vid=D3E34374A0D77D7F&iid=E158A972A605785F&sid=51FE5F6F4794AD16&eid=F434A3C2A19884E7&journal_id=0250-3301&journal_name=环境科学&referenced_num=1&reference_num=23