%0 Journal Article
%T Response of Soil Microbial Community to the Bioremediation of Soil Contaminated with PAHs<br>土壤微生物群落对多环芳烃污染土壤生物修复过程的响应
%A ZHANG Jing
%A LIN Xian-gui
%A LIU Wei-wei
%A YIN Rui
%A <br>张晶
%A 林先贵
%A 刘魏魏
%A 尹睿
%J 环境科学
%D 2012
%I 
%X The diversity of bacterial community in soil contaminated with polycyclic aromatic hydrocarbons (PAHs) was investigated during the plant-microbe remediation enhanced by biosurfactant rhamnolips (RH), using the polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method. The results showed that Shannon-Weaver diversity index was only 3.17 before bioremediation, and increased to 3.24-3.45 after bioremediation, in particular, highest value was found in the treatment of alfalfa (AL) inoculated with arbuscular mycorrhizal fungi (AM) and PAHs-degrading bacteria (DB) among all the treatments. The clustering analysis showed that the similarities of soil bacterial community of AL, AL+RH, AL+AM and AL+AM+RH were above 90%. At the same time, the similarity of AL+DB was much closer to those of the four treatments mentioned above. Additionally, when the bacterial communities of AL+DB+RH, AL+DB+AM and AL+DB+AM+RH were grouped together, the similarities of these three treatments were also higher than 80%. By sequence alignment, it was found that the predominant and characteristic bands in DGGE patterns were closely related with PAHs-degrading bacteria, such as Bacillus, Pseudomonas, Acidobacteria, Sphingmonas, Rhodopseudomonas, Firmicutes, and Methylocytaceae. Application of rhamnolipids in plant-microbe bioremediation not only improved the bioavailability of PAHs, but also had a simultaneous influence on the diversity of soil bacterial community, resulting in the efficient promotion of PAHs removal from soils.
%K PAHs
%K bioremediation
%K microbial community
%K PCR-DGGE
%K biosurfactant
%K arbuscular mycorrhizal fungi
%K PAHs-degrading bacteria<br>多环芳烃
%K 生物修复
%K 微生物群落
%K PCR-DGGE
%K 生物表面活性剂
%K 菌根真菌
%K PAHs-降解菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=669A8D56CCC2B6A1DA723F3CCDF17D3F&yid=99E9153A83D4CB11&vid=27746BCEEE58E9DC&iid=5D311CA918CA9A03&sid=02F9B611052204F2&eid=03BD78E6C243EDEA&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=31