%0 Journal Article %T Comparison of Microbial Community Structure in MBRs Treating Different Wastewater
处理不同废水MBR系统中微生物群落结构的比较 %A ZHANG Bin %A SUN Bao-sheng %A LIU Hui-n %A LIU Xian-hua %A JI Min %A
张斌 %A 孙宝盛 %A 刘慧娜 %A 刘宪华 %A 季民 %J 环境科学 %D 2008 %I %X In order to investigate the microbial community structures in different membrane bioreactors, total bacterial genomic DNA was extracted from biomass in four MBRs treating different wastewater. The microbial community structures were studied by polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE) and cloning-sequencing. Furthermore, and the sequences were used for homology analysis and then two phylogenetic trees were constructed. Results indicate that each MBR owns its specific ecological community after a long-term of operation. Influent wastewater composition has a significant impact on total bacterial community structures. Population diversity, which is in the bioreactor fed with more complex components wastewater, is higher than those of other MBRs. The Shannon Index are 0.77 and 0.78. Proteobacteria and Bacillus, which contain 8 OUTs and 2 OUTs, are the climax communities in the investigable MBRs. The profiles of ammonia-oxidizing bacterial community in the four reactors are very similar. There may exist several modes of nitrification and denitrification in MBR because sequences revealed that there exist multitude nitrifying organisms, in which Nitrosomonas are the most predominant, and two denitrifying bacterium (uncultured Achromobacter sp. and uncultured denitrifying bacterium)in reactors. %K 16S rDNA %K PCR-DGGE
膜生物反应器 %K 微生物群落结构 %K 氨氧化菌 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=AF839B3ED41DD325DB95ACEFCF37BE6E&yid=67289AFF6305E306&vid=771469D9D58C34FF&iid=F3090AE9B60B7ED1&sid=3D7E9628AA346248&eid=EB726ADC575802D8&journal_id=0250-3301&journal_name=环境科学&referenced_num=6&reference_num=19