%0 Journal Article %T Impact of Benzo[a]pyrene the Expression of Mitochondrion-encoded Genes in the Earthworm Eisenia fetida
苯并[a]芘对赤子爱胜蚓线粒体编码基因表达影响研究 %A ZHENG Sen-lin %A SONG Yu-fang %A QIU Xiao-yan %A SUN Tie-heng %A ZHANG Wei %A ML Ackland %A
郑森林 %A 宋玉芳 %A 邱晓燕 %A 孙铁珩 %A 张薇 %A M.L. Ackland %J 环境科学 %D 2008 %I %X The earthworm Eisenia fetida's benzo a] pyrene (BaP) exposure experiments were carried out in artificial soil according to ISO 11268-1:1993. And then the upregulated and downregulated subtractive cDNA libraries were constructed by Clontech PCR-Select cDNA Subtration Kit. From the BaP exposure upregulated subtractive cDNA library, several cDNA segments matched mitochondrion-encoded genes were found, including cytochrome c oxidase subunit I (CO I), subunit II (CO II), subunit Ill (CO III), NADH dehydrogenase subunit 1 (NDH1), and ATP synthase subunit 6. The result indicated BaP and the subsequent oxidative stress disturbed the expression of mitochondrion-encoded genes, and this was potential biomarker for oxidative stress following xenobiotic exposure. %K soil pollution %K benzo(a)pyrene (BaP) %K earthworm Eisenia fetida %K mitochondrion-encoded genes %K suppression subtractive hybridization (SSH)
土壤污染 %K 苯并[a]芘 %K 赤子爱胜蚓 %K 线粒体编码基因 %K 抑制消减杂交 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=FD80D6EE93DC509EA5CD05EA7C4E47C0&yid=67289AFF6305E306&vid=771469D9D58C34FF&iid=0B39A22176CE99FB&sid=AFB2C35009D5D191&eid=21D8CE17EE5EE354&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=27