%0 Journal Article
%T Rosmarinic Acid from Eelgrass Shows Nematicidal and Antibacterial Activities against Pine Wood Nematode and Its Carrying Bacteria
%A Jingyu Wang
%A Xueru Pan
%A Yi Han
%A Daosen Guo
%A Qunqun Guo
%A Ronggui Li
%J Marine Drugs
%D 2012
%I MDPI AG
%R 10.3390/md10122729
%X Pine wilt disease (PWD), a destructive disease for pine trees, is caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus and additional bacteria. In this study, extracts of Zostera marina showed a high nematicidal activity against PWN and some of the bacteria that it carries. Light yellow crystals were obtained from extracts of Z .£¿marina through solvent extraction, followed by chromatography on AB-8 resin and crystallization. The NMR and HPLC analysis showed that the isolated compound was rosmarinic acid (RosA). RosA showed effective nematicidal activity, of which the LC 50 (50% lethal concentration) to PWN at 24 h, 48 h and 72 h was 1.18 mg/g, 1.05 mg/g and 0.95 mg/g, respectively. To get a high yield rate of RosA from Z . marina, single factor experiments and an L 9 (3 4) orthogonal experiment were performed. This extraction process involved 70% ethanol for 3 h at 40 ¡ãC. The extraction dosage was 1:50 (w/v). The highest yield of RosA from Zostera was 3.13 mg/g DW (dried weight). The crude extracts of Zostera marina (10 mg/mL) and RosA (1 mg/mL) also showed inhibitory effects to some bacterial strains carried by PWN: Klebsiella sp., Stenotrophomonas maltophilia, Streptomyces sp. and Pantoea agglomerans. The results of these studies provide clues for preparing pesticide to control PWD from Z . marina.
%K Zostera marina
%K Bursaphelenchus xylophilus
%K rosmarinic acid
%K nematicidal activity
%K anti-bacterial activity
%U http://www.mdpi.com/1660-3397/10/12/2729