%0 Journal Article
%T Effects of Copper (Cu2+ ) and Cu-EDTA Complex on the Induction of HSP70 in the Fish Brain<br>Cu2+和Cu-EDTA对鲫鱼脑组织应激蛋白HSP70诱导的影响
%A SHEN Hu
%A WANG Xiao-rong
%A ZHANG Jing-fei
%A LIU Hui
%A ZHAO Yong-juan
%A <br>沈骅
%A 王晓蓉
%A 张景飞
%A 刘慧
%A 赵永娟
%J 环境科学
%D 2004
%I 
%X The effects of copper (Cu2+) and Cu-EDTA complex on the induction of HSP70 in the fish brain were studied.After the fish (Carassius auratus) were exposed to the experimental concentrations for forty days , the SDS-PAGE and Western Blotting techniques were used to detect the expression level of HSP70 in the fish brain.The results showed that Cu2+ and Cu-EDTA complex can induce the expression of HSP70 significantly.Comparing with the test groups exposed to Cu2+,the Cu-EDTA complex had changed the bioavailability and decreased the toxicity of the heavy metal.It was found that the fish exposed to the concentration of Cu2+ which below the national fishery water quality standard of 0.01mg/L , also had a significant increase in the expression level of HSP70. Therefore, HSP70 as an environmental biomarker can be a more sensitive and rapid indicator than conventional end points in the aquatic ecosystem.
%K heat shock proteins
%K copper and its complex compound
%K biomarker
%K HSP70
%K Western Blotting<br>应激蛋白
%K 铜及其配合物
%K 生物标志物
%K HSP70
%K Western
%K Blotting
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=94D75A2E68196831&yid=D0E58B75BFD8E51C&vid=C5154311167311FE&iid=38B194292C032A66&sid=BB0EA31DB1B01173&eid=C3BF5C58156BEDF0&journal_id=0250-3301&journal_name=环境科学&referenced_num=7&reference_num=10