%0 Journal Article
%T Bacterial Diversity in Sequencing Batch Biofilm Reactor (SBBR) for Landfill Leachate Treatment Using PCR-DGGE<br>PCR-DGGE研究处理垃圾渗滤液序批式生物膜反应器(SBBR)中的细菌多样性
%A XIAO Yong
%A YANG Zhao-hui
%A ZENG Guang-ming
%A MA Yan-he
%A LIU You-sheng
%A WANG Rong-juan
%A XU Zheng-yong
%A <br>肖勇
%A 杨朝晖
%A 曾光明
%A 马延和
%A 刘有胜
%A 王荣娟
%A 徐峥勇
%J 环境科学
%D 2007
%I 
%X For studying the bacterial diversity and the mechanism of denitrification in sequencing bath biofilm reactor (SBBR) treating landfill leachate to provide microbial evidence for technique improvements, total microbial DNA was extracted from samples which were collected from natural landfill leachate and biofilm of a SBBR that could efficiently remove NH+4-N and COD of high concentration. 16S rDNA fragments were amplified from the total DNA successfully using a pair of universal bacterial 16S rDNA primer, GC341F and 907R, and then were used for denaturing gradient gel electrophoresis (DGGE) analysis. The bands in the gel were analyzed by statistical methods and excided from the gel for sequencing, and the sequences were used for homology analysis and then two phylogenetic trees were constructed using DNAStar software. Results indicated that the bacterial diversity of the biofilm in SBBR and the landfill leachate was abundant, and no obvious change of community structure happened during running in the biofilm, in which most bacteria came from the landfill leachate. There may be three different modes of denitrification in the reactor because several different nitrifying bacteria, denitrifying bacteria and anaerobic ammonia oxidation bacteria coexisted in it. The results provided some valuable references for studying microbiological mechanism of denitrification in SBBR.
%K 16S rDNA
%K SBBR
%K PCR
%K DGGE
%K landfill leachate
%K phylogenetic analysis<br>16S
%K rDNA
%K SBBR
%K PCR
%K DGGE
%K 垃圾渗滤液
%K 系统发育分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=BC85E858628321E1B6149A971CCD7637&yid=A732AF04DDA03BB3&vid=D3E34374A0D77D7F&iid=94C357A881DFC066&sid=4814830A427BF9BC&eid=C17A97755771D03C&journal_id=0250-3301&journal_name=环境科学&referenced_num=5&reference_num=20