%0 Journal Article
%T Characteristics of Municipal Sewage Chem-Bioflocculation Treatment Process by Using PCR-DGGE Technology<br>PCR-DGGE技术在城市污水化学生物絮凝处理中的特点
%A WANG Feng
%A FU Yi-gang
%A XIA Si-qing
%A YANG Dian-hai
%A <br>王峰
%A 傅以钢
%A 夏四清
%A 杨殿海
%J 环境科学
%D 2004
%I 
%X It is reported that without cultivation, DNA could be directly extracted from environmental samples with molecular biological methods, such as polymerase chain reaction(PCR) and denaturting gradient gel electrophoresis(DGGE). To analyze the community diversity of activated sludge and bio-film in the municipal sewage, work was done to directly extrude crude DNA from activated sludge and bio-film samples, separate and amplify 16S rDNA by PCR and sequence it with DGGE. The results show the significant microbe community difference between cultivated and uncultivated activated sludge. Further research on the community diversity of two different sewage treatment processes was done and initial discussion on the microbial distribution in the same reactor and microbial structure in different experimental conditions was carried out. The sequences of several 16S rDNA DGGE fragments were determined and some possible bacteria were confirmed in comparision in GeneBank(NCBI). The results show that the PCR-DGGE technology combined with sequences determination is a feasible and efficient method for microorganism analysis in environmental sample.
%K PCR
%K DGGE
%K community diversity
%K activated sludge
%K bio-film<br>聚合酶链式反应
%K 浓度梯度凝胶电泳
%K 微生物结构
%K 活性污泥
%K 生物膜
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=C4C98A8123BFC29F&yid=D0E58B75BFD8E51C&vid=C5154311167311FE&iid=B31275AF3241DB2D&sid=67969BA850333433&eid=9C65ADEB5990B252&journal_id=0250-3301&journal_name=环境科学&referenced_num=17&reference_num=8