%0 Journal Article
%T Preparation of E. coli Antigens with Multi-characters and Antibodies in Water Environment<br>水环境中大肠杆菌多特征抗原及抗体的制备研究
%A WANG N
%A HE Miao
%A SHI Han-chang
%A <br>王娜
%A 何苗
%A 施汉昌
%J 环境科学
%D 2007
%I 
%X In order to establish enzyme-linked immunosorbent assay(ELISA) method to detect E.coli in water environment, E.coli multi-characters antigens in water environment were prepared according to the characters of kinds of E.coli serotypes, including antigen of whole cell, antigen of disrupted whole cell, somatic antigen, flagellar antigen and fimbrial antigen. E.coli polyclonal antibodies were obtained from the New Zealand rabbits immunized with these five antigens, respectively. Antibodies generated in this research are with high titers and good purity, can conjugate with antigens, specifically, stably and strongly. Indirect ELISA shows the titers of antibody of whole cell and antibody of disrupted whole cell are both more than 1×105, and the antibodies are of good quality. Based on these antibodies, we established indirect ELISA method to detect E.coli in water environment. The result shows that the detection limitation could be 104/L.
%K E
%K multi-characters antigen
%K polyclonal antibody
%K water environment
%K immunoassay<br>大肠杆菌
%K 多特征抗原
%K 多克隆抗体
%K 水环境
%K 免疫检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=BC85E858628321E11EF7DB277F80BC3B&yid=A732AF04DDA03BB3&vid=D3E34374A0D77D7F&iid=94C357A881DFC066&sid=4442BFF895478018&eid=D542ADE6529F4059&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=20