%0 Journal Article
%T Community of Activated Sludge Based on Different Targeted Sequence of 16S rDNA by Denaturing Gradient Gel Electrophoresis<br>不同16SrDNA靶序列对DGGE分析活性污泥群落的影响
%A XING De-feng
%A REN Nan-qi
%A SONG Jia-xiu
%A QU Min
%A XU Xiang-ling
%A <br>邢德峰
%A 任南琪
%A 宋佳秀
%A 曲敏
%A 徐香玲
%J 环境科学
%D 2006
%I 
%X In order to realize effect of different sets of universal primers on the analysis of microbial community of activated sludge based on targeted sequence of 16S rDNA and employ effectively denaturing gradient gel electrophoresis(DGGE),activated sludge were obtained from the continuous stirred tank reactor(CSTR),16S rDNA fragments were amplified with there primer sets(341f/534r,(968f/1?401r) and 341f/926r),and the diversity and dynamics of microbial communities were investigated by DGGE.The results indicated that community diversity and dynamics was obviously different based different sets of universal primers by DGGE.Separated patterns of the targeted sequence of primer 341f/534r and(968f/1?401r) were better than of(341f/926r.) The similarity of communities between S2 and S3 was high in the DGGE profiles with primer 341f/534r and 341f/926r,the similarity of communities between S1 and S2 was high in the DGGE profiles with primer(968f/1?401r,) it demonstrated that the similarity and dynamics of communities was different each other based different sets of universal primers.In the DGGE profiles, bands and diversity from primer 341f/534r were most,bands and diversity from primer 341f/926r were least.Thereby,while activated sludge was analyzed by DGGE,primers 341f/534r and(968f/1?401r) were more effective than 341f/926r.
%K DGGE
%K 16S rDNA
%K activated sludge
%K community dynamics
%K community diversity<br>DGGE
%K 16SrDNA
%K 活性污泥
%K 群落动态
%K 群落多样性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=A9D2858E28E15699&yid=37904DC365DD7266&vid=DB817633AA4F79B9&iid=DF92D298D3FF1E6E&sid=AFD02B86BFB3C7FC&eid=1658FDEF8AE04907&journal_id=0250-3301&journal_name=环境科学&referenced_num=4&reference_num=11