%0 Journal Article
%T Construction of Plasmid with Green Fluorescent Protein Gene for Analysis of Yeasts in Yeast Wastewater Treatment<br>用于废水处理系统中目标酵母动态解析的绿色荧光蛋白基因质粒构建
%A HAN Yun
%A XING Xin-hui
%A YANG Min
%A LIU Min-sheng
%A LOU Kai
%A <br>韩云
%A 邢新会
%A 杨敏
%A 刘敏胜
%A 娄恺
%J 环境科学
%D 2004
%I 
%X Green fluorescent protein(GFP) can be utilized in analysis of the characteristics and distribution of a targeted strain in microbial communities. This study is the first step to establish a dynamic yeast monitoring technique in a wastewater treatment system using yeast by constructing a fluorescent yeast containing gfp gene. The gfp gene was inserted into pACT1-URA3, a powerful plasmid for introducing a foreign gene into Candida boidinii, and then transformed into E coli JM109. The gfp gene was expressed, though not very highly. The results of the electrophoresis and polymerase chain reaction suggested that the newly constructed plasmid containing gfp gene might not exist in free form in the cells, but in some special way such as interaction with the chromosome.
%K green fluorescent protein
%K yeast wastewater treatment
%K sludge bulking
%K polymerase chain reaction<br>绿色荧光蛋白
%K 酵母菌处理系统
%K 污泥膨胀
%K 聚合酶链反应
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=5DB2A419F737E956&yid=D0E58B75BFD8E51C&vid=C5154311167311FE&iid=CA4FD0336C81A37A&sid=1371F55DA51B6E64&eid=1AE5323881A5ECDC&journal_id=0250-3301&journal_name=环境科学&referenced_num=2&reference_num=12